Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens

Virol J. 2024 Jul 11;21(1):156. doi: 10.1186/s12985-024-02430-x.

Abstract

Objectives: The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed.

Methods: Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method.

Results: The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively.

Conclusions: The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens.

Keywords: Fluorescent probe melting curve PCR; Molecular detection; Respiratory pathogen.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Child
  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Molecular Diagnostic Techniques* / methods
  • Nasopharynx* / microbiology
  • Nasopharynx* / virology
  • Prospective Studies
  • Respiratory Tract Infections* / diagnosis
  • Respiratory Tract Infections* / microbiology
  • Respiratory Tract Infections* / virology
  • Sensitivity and Specificity
  • Specimen Handling / methods
  • Sputum* / microbiology
  • Sputum* / virology
  • Viruses / classification
  • Viruses / genetics
  • Viruses / isolation & purification
  • Young Adult