Integrating HexNAcQuest with Glycoproteomics Data Analysis Software to Distinguish HexNAc Isomers on Peptides

Chunyan Hou; Weiyu Li; Yaoxiang Li; Junfeng Ma

doi:10.1007/978-1-0716-4007-4_5

Integrating HexNAcQuest with Glycoproteomics Data Analysis Software to Distinguish HexNAc Isomers on Peptides

Methods Mol Biol. 2024:2836:67-76. doi: 10.1007/978-1-0716-4007-4_5.

Authors

Chunyan Hou¹, Weiyu Li^{1

2}, Yaoxiang Li¹, Junfeng Ma³

Affiliations

¹ Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA.
² Department of Applied Mathematics and Statistics, Johns Hopkins University, Baltimore, MD, USA.
³ Department of Oncology, Lombardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA. [email protected].

PMID: 38995536
DOI: 10.1007/978-1-0716-4007-4_5

Abstract

Recently, HexNAcQuest was developed to help distinguish peptides modified by HexNAc isomers, more specifically O-linked β-N-acetylglucosamine (O-GlcNAc) and O-linked α-N-acetylgalactosamine (O-GalNAc, Tn antigen). To facilitate its usage (particularly for datasets from glycoproteomics studies), herein we present a detailed protocol. It describes example cases and procedures for which users might need to use HexNAcQuest to distinguish these two modifications.

Keywords: Glycoproteomics; HexNAc; HexNAcQuest; O-GlcNAc; Tn antigen.

MeSH terms

Acetylgalactosamine / chemistry
Acetylglucosamine / chemistry
Glycomics* / methods
Isomerism
Peptides* / chemistry
Proteomics / methods
Software*

Substances

Acetylglucosamine
Peptides
Acetylgalactosamine