Methodological approaches to studying phase separation and HIV-1 replication: Current and future perspectives

Andrew J Mouland; Bao-An Chau; Vladimir N Uversky

doi:10.1016/j.ymeth.2024.07.002

Methodological approaches to studying phase separation and HIV-1 replication: Current and future perspectives

Methods. 2024 Sep:229:147-155. doi: 10.1016/j.ymeth.2024.07.002. Epub 2024 Jul 15.

Authors

Andrew J Mouland¹, Bao-An Chau², Vladimir N Uversky³

Affiliations

¹ Department of Medicine, McGill University, Montreal, Quebec, Canada. Electronic address: [email protected].
² Department of Medicine, McGill University, Montreal, Quebec, Canada.
³ Department of Molecular Medicine and Byrd Alzheimer's Research Institute, Morsani College of Medicine, University of South Florida, Tampa, FL, USA. Electronic address: [email protected].

PMID: 39002735
DOI: 10.1016/j.ymeth.2024.07.002

Abstract

This article reviews tried-and-tested methodologies that have been employed in the first studies on phase separating properties of structural, RNA-binding and catalytic proteins of HIV-1. These are described here to stimulate interest for any who may want to initiate similar studies on virus-mediated liquid-liquid phase separation. Such studies serve to better understand the life cycle and pathogenesis of viruses and open the door to new therapeutics.

Keywords: Capsid; Condensation; Fluorescence recovery after photobleaching; HIV-1; In-cell condensation; Intrinsic disorder; Liquid–liquid phase separation; Nucleocapsid; Virus assembly.

Publication types

Review

MeSH terms

HIV Infections / virology
HIV-1* / genetics
HIV-1* / physiology
Humans
Phase Separation
RNA, Viral / genetics
Virus Replication*

Substances

RNA, Viral