Validated Methods for Effective Decontamination and Inactivation of Rift Valley Fever Virus

Methods Mol Biol. 2024:2824:81-89. doi: 10.1007/978-1-0716-3926-9_6.

Abstract

The Rift Valley fever virus (RVFV) is an arthropod-borne, zoonotic, hemorrhagic fever virus that can cause severe diseases both in livestock and humans. The spread of RVFV in areas previously considered as non-endemic together with the absence of licensed vaccines for use in humans and animals poses a major health and economic threat worldwide. It is therefore crucial to make major progresses in our understanding and management of this virus and its zoonosis. RVFV is considered a bioterrorism pathogen, and, thus, only a few institutes, facilities, and personnel are legally authorized to detain it and handle it. Moreover, this virus must be manipulated in a biosafety level 3 (BSL3) laboratory following strict biosafety protocols to ensure that biosecurity's highest standards are met. Only certain attenuated strains such as the MP12 strain can be handled in BSL2 laboratories, depending on the country considered. To assist researchers in working with RVFV in the safest possible conditions, this chapter presents validated methods for effective RVFV decontamination and inactivation.

Keywords: BSL3; Decontamination; Inactivation; Rift Valley fever virus.

MeSH terms

  • Animals
  • Chlorocebus aethiops
  • Containment of Biohazards / methods
  • Decontamination* / methods
  • Humans
  • Rift Valley Fever / prevention & control
  • Rift Valley Fever / transmission
  • Rift Valley Fever / virology
  • Rift Valley fever virus*
  • Vero Cells
  • Virus Inactivation*