SARS-CoV-2 S1 subunit produces a protracted priming of the neuroinflammatory, physiological, and behavioral responses to a remote immune challenge: A role for corticosteroids

Brain Behav Immun. 2024 Oct:121:87-103. doi: 10.1016/j.bbi.2024.07.034. Epub 2024 Jul 21.

Abstract

Long COVID is a major public health consequence of COVID-19 and is characterized by multiple neurological and neuropsychatric symptoms. SARS-CoV-2 antigens (e.g., spike S1 subunit) are found in the circulation of Long COVID patients, have been detected in post-mortem brain of COVID patients, and exhibit neuroinflammatory properties. Considering recent observations of chronic neuroinflammation in Long COVID patients, the present study explores the idea that antigens derived from SARS-CoV-2 might produce a long-term priming or sensitization of neuroinflammatory processes, thereby potentiating the magnitude and/or duration of the neuroinflammatory response to future inflammatory insults. Rats were administered S1 or vehicle intra-cisterna magna and 7d later challenged with vehicle or LPS. The neuroinflammatory, physiological, and behavioral responses to LPS were measured at various time points post-LPS. We found that prior S1 treatment potentiated many of these responses to LPS suggesting that S1 produces a protracted priming of these processes. Further, S1 produced a protracted reduction in basal brain corticosteroids. Considering the anti-inflammatory properties of corticosteroids, these findings suggest that S1 might disinhibit innate immune processes in brain by reducing anti-inflammatory drive, thereby priming neuroinflammatory processes. Given that hypocortisolism is observed in Long COVID, we propose that similar S1-induced innate immune priming processes might play role in the pathophysiology of Long COVID.

Keywords: Corticosteroids; Long COVID; Microglia; Neuroinflammation; Priming; SARS-CoV-2.

MeSH terms

  • Adrenal Cortex Hormones / pharmacology
  • Animals
  • Brain / drug effects
  • Brain / immunology
  • Brain / metabolism
  • COVID-19* / immunology
  • Inflammation / immunology
  • Inflammation / metabolism
  • Lipopolysaccharides* / pharmacology
  • Male
  • Neuroinflammatory Diseases* / immunology
  • Rats
  • Rats, Sprague-Dawley
  • SARS-CoV-2* / immunology
  • Spike Glycoprotein, Coronavirus* / immunology
  • Spike Glycoprotein, Coronavirus* / metabolism

Substances

  • Lipopolysaccharides
  • Spike Glycoprotein, Coronavirus
  • Adrenal Cortex Hormones
  • spike protein, SARS-CoV-2