Combined Flow-Fluorescence in situ hybridization to HHV-8 and EBV reveals the viral heterogeneity of primary effusion lymphoma

Romain Stammler; Lauriane Vacher; Benjamin Fournier; Pierre Lemaire; Clémentine Chauvel; Marc-Antoine Silvestrini; Silène Knapp; Grégoire Martin de Frémont; Véronique Meignin; Maud Salmona; Jérôme Legoff; Anthony Vanjak; Bertrand Dunogué; Fanny Urbain; Olivier Lambotte; Nicolas Noël; Laurence Gérard; Eric Oksenhendler; Lionel Galicier; Sylvain Latour; David Boutboul

doi:10.1002/jmv.29836

Combined Flow-Fluorescence in situ hybridization to HHV-8 and EBV reveals the viral heterogeneity of primary effusion lymphoma

J Med Virol. 2024 Aug;96(8):e29836. doi: 10.1002/jmv.29836.

Authors

Romain Stammler¹, Lauriane Vacher², Benjamin Fournier³, Pierre Lemaire⁴, Clémentine Chauvel⁴, Marc-Antoine Silvestrini^{1

3}, Silène Knapp¹, Grégoire Martin de Frémont¹, Véronique Meignin^{5

6}, Maud Salmona⁷, Jérôme Legoff⁷, Anthony Vanjak¹, Bertrand Dunogué⁸, Fanny Urbain⁹, Olivier Lambotte⁹, Nicolas Noël⁹, Laurence Gérard^{2

5}, Eric Oksenhendler^{2

5}, Lionel Galicier^{2

5}, Sylvain Latour³, David Boutboul^{1

3

5

10}

Affiliations

¹ Inserm U976 HIPI, Institut Universitaire d'Hématologie, Université Paris Cité, Paris, France.
² Department of Clinical Immunology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.
³ Laboratory of Lymphocyte Activation and Susceptibility to EBV infection, INSERM UMR 1163, Imagine Institute, Université Paris Cité, Paris, France.
⁴ Laboratory of Haematology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.
⁵ National Reference Center for Castleman disease, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.
⁶ Pathology Department, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.
⁷ Laboratory of Virology, Hôpital Saint Louis, AP-HP, Université Paris Cité, Paris, France.
⁸ Department of Internal Medicine, Hôpital Cochin, AP-HP, Université Paris Cité, Paris, France.
⁹ Department of Internal Medicine, Hôpital Bicêtre, AP-HP, Le Kremlin-Bicêtre, Université Paris Saclay, Le Kremlin-Bicetre, France.
¹⁰ Department of Hematology, Hôpital Cochin, AP-HP, Université Paris Cité, Paris, France.

PMID: 39078052
DOI: 10.1002/jmv.29836

Abstract

Primary effusion lymphoma (PEL) is a rare B-cell non-Hodgkin lymphoma associated with Kaposi Sarcoma-associated herpesvirus (KSHV/HHV8) infection. Lymphoma cells are coinfected with Epstein-Barr virus (EBV) in 60-80% of cases. Tools allowing a reliable PEL diagnosis are lacking. This study reports PEL diagnosis in 4 patients using a Flow-Fluorescence in situ hybridization (FlowFISH) technique that allowed detection of differentially expressed EBV and HHV8 transcripts within the same sample, revealing viral heterogeneity of the disease. Moreover, infected cells exhibited variable expressions of CD19, CD38, CD40, and CD138. Therefore, FlowFISH is a promising tool to diagnose and characterize complex viral lymphoproliferations.

Keywords: EBV; Flow‐Fluorescence in situ hybridization; HHV8; KSHV; primary effusion lymphoma.

Publication types

Case Reports

MeSH terms

Aged
Aged, 80 and over
Epstein-Barr Virus Infections / complications
Epstein-Barr Virus Infections / virology
Female
Herpesviridae Infections / diagnosis
Herpesviridae Infections / virology
Herpesvirus 4, Human* / genetics
Herpesvirus 4, Human* / isolation & purification
Herpesvirus 8, Human* / genetics
Herpesvirus 8, Human* / isolation & purification
Humans
In Situ Hybridization, Fluorescence* / methods
Lymphoma, Primary Effusion* / virology
Male
Middle Aged