Generation of induced pluripotent stem cells from an HLA-B27 positive ankylosing spondylitis patient with syndesmophyte formation

Wan-Hao Tsai; Yen-Chun Lin; Shih-Hsun Lan; Yu-Heng Lin; Chun-Hsiung Chen; Song-Chou Hsieh; Chin-Hsiu Liu

doi:10.1016/j.scr.2024.103508

Generation of induced pluripotent stem cells from an HLA-B27 positive ankylosing spondylitis patient with syndesmophyte formation

Stem Cell Res. 2024 Oct:80:103508. doi: 10.1016/j.scr.2024.103508. Epub 2024 Jul 25.

Authors

Wan-Hao Tsai¹, Yen-Chun Lin², Shih-Hsun Lan², Yu-Heng Lin², Chun-Hsiung Chen³, Song-Chou Hsieh⁴, Chin-Hsiu Liu⁵

Affiliations

¹ Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan; Division of Immunology and Rheumatology, Fu Jen Catholic University Hospital, New Taipei City, Taiwan.
² Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan.
³ Division of Allergy, Immunology and Rheumatology, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan; School of Medicine, Tzu Chi University, Hualien, Taiwan.
⁴ Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan; College of Medicine, National Taiwan University, Taipei, Taiwan.
⁵ Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, Taiwan; Division of Allergy, Immunology & Rheumatology, Department of Internal Medicine, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan; College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Medical Education and Research, National Taiwan University Hospital Yunlin Branch, Yunlin, Taiwan. Electronic address: [email protected].

PMID: 39079398
DOI: 10.1016/j.scr.2024.103508

Abstract

Human leukocyte antigen (HLA)-B27 is the genetic marker for ankylosing spondylitis (AS). Here, we generated induced pluripotent stem cells (iPSCs) from peripheral blood mononuclear cells of a male AS patient carrying HLA-B27 with syndesmophyte formation by using the Sendai-virus delivery system. The resulting iPSCs had a normal karyotype, expressed pluripotent markers, and could differentiate into three germ layers. This cellular model will provide a platform for studying pathological mechanisms of new bone formation in HLA-B27 positive AS patients.

MeSH terms

Adult
Cell Differentiation
HLA-B27 Antigen* / genetics
HLA-B27 Antigen* / metabolism
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear / metabolism
Male
Spondylitis, Ankylosing* / genetics
Spondylitis, Ankylosing* / metabolism
Spondylitis, Ankylosing* / pathology

Substances

HLA-B27 Antigen