Suppression of B cell development and antibody responses in mice with polyclonal rabbit and monoclonal rat anti-IgM antibodies. I. Characterization of the suppressed state

Exp Cell Biol. 1985;53(6):301-13. doi: 10.1159/000163327.

Abstract

Mice treated from birth with polyclonal, crude or affinity purified rabbit or monoclonal rat anti-mouse IgM antibodies [b-7-6 and C-2-23: Eur. J. Immunol. 14: 753-757, 1984] were found to be heavily suppressed with respect to B-cell activities. Crude or affinity purified rabbit or monoclonal rat anti-mouse IgM gave comparable results as follows: serum IgM was below detectable levels; serum IgG was reduced to about 1-3% of normal levels; free anti-IgM was always detectable; IgM and/or kappa-light-chain positive cells as well as IgM-secreting cells were absent in various lymphoid organs; the B-cell mitogen lipopolysaccharide was unable to induce proliferative responses; primary antibody responses could not be induced against sheep red blood cells and phosphorylcholine; lymphoid organs were reduced in size and B-cell areas were not populated with lymphocytes; besides a 40% reduction in absolute lymphocyte numbers in the blood, we found increased platelet counts and a 10% eosinophilia in anti-IgM-treated mice.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic / administration & dosage*
  • Antibodies, Anti-Idiotypic / physiology*
  • Antibodies, Monoclonal / administration & dosage*
  • Antibody Formation
  • B-Lymphocytes / immunology*
  • Bone Marrow Cells
  • Hemolytic Plaque Technique
  • Immune Sera / administration & dosage
  • Immune Tolerance*
  • Immunoglobulin G / biosynthesis
  • Immunoglobulin G / metabolism
  • Immunoglobulin M / biosynthesis
  • Immunoglobulin M / immunology
  • Immunoglobulin M / physiology*
  • Lymph Nodes / cytology
  • Lymphocyte Activation*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Rabbits
  • Rats
  • Spleen / cytology

Substances

  • Antibodies, Anti-Idiotypic
  • Antibodies, Monoclonal
  • Immune Sera
  • Immunoglobulin G
  • Immunoglobulin M
  • anti-IgM