The Caenorhabditis elegans germline is arranged spatiotemporally and is therefore a powerful model system for the interrogation of meiotic molecular dynamics. Coupling this property with the temporal control that the auxin-inducible degron (AID) system allows can unveil new/unappreciated roles for critical meiotic factors in specific germline regions. Here we describe a widely used approach for the introduction of degron tags to specific targets and provide a procedure for applying the AID system to C. elegans meiotic DSB repair dynamics in the germline.
Keywords: Auxin; Caenorhabditis elegans; Crossovers; Germline; Meiosis.
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.