Distinctive duodenal microbiomes and bile acid profiles in duodenal tumor patients revealed by prospective observational study

Sci Rep. 2024 Aug 12;14(1):18705. doi: 10.1038/s41598-024-69820-7.

Abstract

The incidence of duodenal tumors (DTs) is increasing. However, the mechanisms underlying its development remain unclear. Environmental factors, including the microbiome and bile acids (BAs), are believed to influence tumor development. Therefore, we conducted a single-center, prospective, observational study to investigate the potential differences between patients with DTs and healthy controls (HCs) based on these factors. In addition, the BAs in the duodenal fluid were measured using liquid chromatography-tandem mass spectrometry. We recruited 41 patients and performed 16S rRNA-seq. There was no difference in the observed ASVs or PCoA plot of Bray-Curtis dissimilarity between the DTs and HCs. The lithocholic acid concentration was significantly lower in the DT group than in the control group. The ratio of CDCA to LCA was significantly higher in patients with DTs. No significant differences in microbiota were observed between DTs and HCs. In patients with DTs, the lithocholic acid concentration in duodenal was significantly lower than in HCs.

Publication types

  • Observational Study

MeSH terms

  • Adult
  • Aged
  • Bile Acids and Salts* / metabolism
  • Case-Control Studies
  • Duodenal Neoplasms* / metabolism
  • Duodenal Neoplasms* / microbiology
  • Duodenum* / metabolism
  • Duodenum* / microbiology
  • Female
  • Gastrointestinal Microbiome*
  • Humans
  • Lithocholic Acid / metabolism
  • Male
  • Microbiota
  • Middle Aged
  • Prospective Studies
  • RNA, Ribosomal, 16S* / genetics

Substances

  • Bile Acids and Salts
  • RNA, Ribosomal, 16S
  • Lithocholic Acid