Morin attenuated the global cerebral ischemia via antioxidant, anti-inflammatory, and antiapoptotic mechanisms in rats

Metab Brain Dis. 2024 Oct;39(7):1323-1334. doi: 10.1007/s11011-024-01410-y. Epub 2024 Aug 13.

Abstract

Global cerebral ischemia is one of the major causes of memory and cognitive impairment. Hyperactivation of acetylcholine esterase (AChE), oxidative stress, and inflammation are reported to cause memory and cognitive impairment in global cerebral ischemia. Morin, a flavonoid, is reported to have neuroprotective properties through its antioxidant and anti-inflammatory in multiple neurological diseases. However, its neuroprotective effects and memory and cognition enhancement have not yet been investigated. In the present study, we have determined the memory and cognition, and neuroprotective activity of Morin in bilateral common carotid artery occlusion and reperfusion (BCCAO/R) in Wistar rats. We found that Morin treatment significantly improved motor performance like grip strength and rotarod. Further, Morin improved memory and cognition in BCCAO rats by decreasing the AchE enzyme activity and enhancing the acetylcholine (Ach) levels. Additionally, Morin exhibited neuroprotection by ameliorating oxidative stress, neuroinflammation, and apoptosis in BCCAO rats. These findings confirm that Morin could enhance memory and cognition by ameliorating AchE activity, oxidative stress, neuroinflammation, and apoptosis in global cerebral ischemia. Therefore, Morin could be a promising neuroprotective and memory enhancer against global cerebral ischemic injury.

Keywords: Acetylcholine esterase; Apoptosis; BCCAO; Global cerebral ischemia; Memory impairment; Morin.

MeSH terms

  • Acetylcholinesterase / metabolism
  • Animals
  • Anti-Inflammatory Agents* / pharmacology
  • Anti-Inflammatory Agents* / therapeutic use
  • Antioxidants* / pharmacology
  • Antioxidants* / therapeutic use
  • Apoptosis* / drug effects
  • Brain Ischemia* / drug therapy
  • Brain Ischemia* / metabolism
  • Cognition / drug effects
  • Flavones
  • Flavonoids* / pharmacology
  • Flavonoids* / therapeutic use
  • Male
  • Memory / drug effects
  • Neuroprotective Agents* / pharmacology
  • Neuroprotective Agents* / therapeutic use
  • Oxidative Stress* / drug effects
  • Rats
  • Rats, Wistar*
  • Reperfusion Injury / drug therapy
  • Reperfusion Injury / metabolism
  • Reperfusion Injury / prevention & control

Substances

  • morin
  • Flavonoids
  • Antioxidants
  • Neuroprotective Agents
  • Anti-Inflammatory Agents
  • Acetylcholinesterase
  • Flavones