PCPE-1, a brown adipose tissue-derived cytokine, promotes obesity-induced liver fibrosis

EMBO J. 2024 Nov;43(21):4846-4869. doi: 10.1038/s44318-024-00196-0. Epub 2024 Aug 19.

Abstract

Metabolic dysfunction-associated steatohepatitis (MASH, previously termed non-alcoholic steatohepatitis (NASH)), is a major complication of obesity that promotes fatty liver disease. MASH is characterized by progressive tissue fibrosis and sterile liver inflammation that can lead to liver cirrhosis, cancer, and death. The molecular mechanisms of fibrosis in MASH and its systemic control remain poorly understood. Here, we identified the secreted-type pro-fibrotic protein, procollagen C-endopeptidase enhancer-1 (PCPE-1), as a brown adipose tissue (BAT)-derived adipokine that promotes liver fibrosis in a murine obesity-induced MASH model. BAT-specific or systemic PCPE-1 depletion in mice ameliorated liver fibrosis, whereas, PCPE-1 gain of function in BAT enhanced hepatic fibrosis. High-calorie diet-induced ER stress increased PCPE-1 production in BAT through the activation of IRE-1/JNK/c-Fos/c-Jun signaling. Circulating PCPE-1 levels are increased in the plasma of MASH patients, suggesting a therapeutic possibility. In sum, our results uncover PCPE-1 as a novel systemic control factor of liver fibrosis.

Keywords: BATokine; Fibrosis; MASH; Obesity; PCPE-1.

MeSH terms

  • Adipose Tissue, Brown* / metabolism
  • Adipose Tissue, Brown* / pathology
  • Animals
  • Cytokines / metabolism
  • Disease Models, Animal
  • Endoplasmic Reticulum Stress
  • Humans
  • Liver Cirrhosis* / genetics
  • Liver Cirrhosis* / metabolism
  • Liver Cirrhosis* / pathology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Non-alcoholic Fatty Liver Disease / etiology
  • Non-alcoholic Fatty Liver Disease / metabolism
  • Non-alcoholic Fatty Liver Disease / pathology
  • Obesity* / complications
  • Obesity* / metabolism
  • Obesity* / pathology
  • Signal Transduction

Substances

  • Cytokines