Elevated C-Reactive Protein in Older Men With Chronic Pain: Association With Plasma Amyloid Levels and Hippocampal Volume

J Gerontol A Biol Sci Med Sci. 2024 Nov 1;79(11):glae206. doi: 10.1093/gerona/glae206.

Abstract

Background: Chronic pain leads to tau accumulation and hippocampal atrophy, which may be moderated through inflammation. In older men, we examined associations of chronic pain with Alzheimer's disease (AD)-related plasma biomarkers and hippocampal volume as moderated by systemic inflammation.

Methods: Participants were men without dementia. Chronic pain was defined as moderate-to-severe pain in 2+ study waves at average ages 56, 62, and 68. At age 68, we measured plasma amyloid-beta (Aβ42, n = 871), Aβ40 (n = 887), total tau (t-tau, n = 841), and neurofilament light chain (NfL, n = 915), and serum high-sensitivity C-reactive protein (hs-CRP, n = 968), a marker of systemic inflammation. A subgroup underwent structural MRI to measure hippocampal volume (n = 385). Analyses adjusted for medical morbidities, depressive symptoms, and opioid use.

Results: Chronic pain was related to higher Aβ40 (β = 0.25, p = .009), but hs-CRP was unrelated to AD-related biomarkers (ps > .05). There was a significant interaction such that older men with both chronic pain and higher levels of hs-CRP had higher levels of Aβ42 (β = 0.36, p = .001) and Aβ40 (β = 0.29, p = .003). Chronic pain and hs-CRP did not interact to predict levels of Aβ42/Aβ40, t-tau, or NfL. Furthermore, there were significant interactions such that Aβ42 and Aβ40 were associated with lower hippocampal volume, particularly when levels of hs-CRP were elevated (hs-CRP × Aβ42: β = -0.19, p = .002; hs-CRP × Aβ40: β = -0.21, p = .001), regardless of chronic pain status.

Conclusions: Chronic pain was associated with higher plasma Aβ, especially when hs-CRP was also elevated. Higher hs-CRP and Aβ levels were both related to smaller hippocampal volumes. Chronic pain, when accompanied by systemic inflammation, may elevate the risk of neurodegeneration in AD-vulnerable regions.

Keywords: Amyloid-beta; Chronic pain; Hippocampus; Inflammation; Plasma biomarkers.

MeSH terms

  • Aged
  • Alzheimer Disease / blood
  • Alzheimer Disease / pathology
  • Amyloid beta-Peptides* / blood
  • Biomarkers* / blood
  • C-Reactive Protein* / analysis
  • C-Reactive Protein* / metabolism
  • Chronic Pain* / blood
  • Hippocampus* / diagnostic imaging
  • Hippocampus* / pathology
  • Humans
  • Inflammation / blood
  • Magnetic Resonance Imaging*
  • Male
  • Middle Aged
  • Neurofilament Proteins / blood
  • Organ Size
  • Peptide Fragments / blood
  • tau Proteins* / blood

Substances

  • C-Reactive Protein
  • Amyloid beta-Peptides
  • Biomarkers
  • tau Proteins
  • Neurofilament Proteins
  • Peptide Fragments
  • neurofilament protein L