Objective: To explore whether thiotert treatment can inhibit proliferation and induce apoptosis in myelodysplastic syndromes (MDS) cells.
Methods: CCK-8 assay was used for determining the cytotoxicity of thiotert to MDS cell line SKM-1 and the reversal effect of GSH, NAC, and Z-VAD-FMK on thiotert-induced inhibition of cell viability. EdU assay was deployed to detect the cell proliferation ability. Intracellular reactive oxygen species (ROS) was measured by flow cytometry after DCFH-DA staining. The expression of DNA damage- and apoptosis-related proteins was detected by Western blot.
Results: Thiotert treatment significantly suppressed the cell viability and proliferation ability in SKM-1 cells. A large amount of ROS generation and markedly elevated C-PARP, C-Caspase 3, and γ-H2AX were observed after thiotert administration, while BCL-2 was significantly decreased. In addition, GSH, NAC, and Z-VAD-FMK were able to mitigate the cytotoxicity of thiotert on SKM-1 cells.
Conclusion: Thiotert can promote MDS cell apoptosis by mediating ROS production and pro-apoptotic proteins expression.
题目: 6-异丙基双硫-2′-脱氧鸟苷通过氧化应激诱导骨髓增生异常综合征细胞凋亡.
目的: 研究6-异丙基双硫-2′-脱氧鸟苷（thiotert）对骨髓增生异常综合征（MDS）细胞SKM-1增殖及凋亡的影响。.
方法: 采用CCK-8法检测thiotert对SKM-1细胞活力的抑制及GSH、NAC和Z-VAD-FMK对thiotert细胞毒性的逆转作用；EdU细胞增殖检测试剂盒检测细胞增殖情况；流式细胞术检测细胞内的活性氧（ROS）水平；Western blot检测DNA损伤及凋亡相关蛋白的表达。.
结果: thiotert显著抑制了SKM-1细胞的增殖能力，诱导SKM-1细胞中ROS水平升高；thiotert处理导致SKM-1细胞内C-PARP、C-Caspase 3和γ-H2AX蛋白表达上调，而BCL-2显著下调；抗氧化剂GSH和NAC及凋亡抑制剂Z-VAD-FMK能部分逆转thiotert对细胞活力的抑制作用。.
结论: thiotert能够通过介导ROS的产生和促凋亡蛋白的表达来促进MDS细胞发生凋亡。.
Keywords: myelodysplastic syndromes; thiotert; reactive oxygen species; apoptosis.