Metabolic consequences of interesterified palm oil and PCB-126 co-exposure in C57BL/6 mice

Food Chem Toxicol. 2024 Oct:192:114965. doi: 10.1016/j.fct.2024.114965. Epub 2024 Aug 27.

Abstract

Metabolic dysfunction-associated steatotic liver disease (MASLD) is defined as morphofunctional changes in the liver. Studies have shown that Westernized eating patterns and environmental pollutants can directly induce the development of MASLD. This study evaluates the effect of co-exposure to interesterified palm oil (IPO) and 3,3',4,4',5-pentachlorobiphenyl (PCB-126) on the progression of MASLD in an animal model. C57BL/6 mice were fed IPO and co-exposed to PCB-126 for ten weeks. The co-exposure led to an imbalance in carbohydrate metabolism, increased systemic inflammation markers, and morphofunctional changes in the liver. These liver changes included the presence of inflammatory cells, fibrosis, alterations in aspartate transaminase (AST) and alanine transaminase (ALT) enzymes, and imbalance in gene expression related to fatty acid β-oxidation, de novo lipogenesis, mitochondrial dynamics, and endoplasmic reticulum stress. Separate exposures to IPO and PCB-126 affected metabolism and MASLD progression. Nutritional and lifestyle factors may potentiate the onset and severity of MASLD.

Keywords: Endoplasmic reticulum stress; Hepatic steatosis; Inflammatory markers; Metabolic dysfunction; Mitochondrial dysfunction.

MeSH terms

  • Animals
  • Endoplasmic Reticulum Stress / drug effects
  • Environmental Pollutants / toxicity
  • Fatty Liver / chemically induced
  • Fatty Liver / metabolism
  • Liver* / drug effects
  • Liver* / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL*
  • Palm Oil*
  • Polychlorinated Biphenyls* / toxicity

Substances

  • Palm Oil
  • Polychlorinated Biphenyls
  • 3,4,5,3',4'-pentachlorobiphenyl
  • Environmental Pollutants