Assaying D-Alanine Racemase in Lactic Acid Bacteria Using NADH Oxidoreduction Enzymic System

Methods Mol Biol. 2024:2851:115-123. doi: 10.1007/978-1-0716-4096-8_10.

Abstract

A notable characteristic of amino acids is their optical isomerism, existing as L-form and D-form. Proteins are composed exclusively of L-form amino acids. However, recently, it is reported that D-alanine is evaluated particularly highly in terms of sensory evaluation. D-body amino acids convert L-body amino acid proteolysis from a substrate such as foods during fermentation of lactic acid bacteria. This chapter presents a description of methods used for D-alanine racemase assays in the solution producing by lactic acid bacteria (LAB) using D-amino acid oxidase and lactic acid dehydrogenase via a NADH oxidoreduction system.

Keywords: Alanine racemase; D-alanine; D-amino acid oxidase; NADH; Oxidoreduction system.

MeSH terms

  • Alanine Racemase* / genetics
  • Alanine Racemase* / metabolism
  • D-Amino-Acid Oxidase / metabolism
  • Enzyme Assays / methods
  • L-Lactate Dehydrogenase / metabolism
  • Lactobacillales / enzymology
  • Lactobacillales / metabolism
  • NAD* / metabolism
  • Oxidation-Reduction*

Substances

  • NAD
  • Alanine Racemase
  • D-Amino-Acid Oxidase
  • L-Lactate Dehydrogenase