Low-Frequency Coherent Raman Imaging Robust to Optical Scattering

David R Smith; Jesse W Wilson; Siddarth Shivkumar; Hervé Rigneault; Randy A Bartels

doi:10.1021/cbmi.4c00020

Low-Frequency Coherent Raman Imaging Robust to Optical Scattering

Chem Biomed Imaging. 2024 Jul 8;2(8):584-591. doi: 10.1021/cbmi.4c00020. eCollection 2024 Aug 26.

Authors

David R Smith¹, Jesse W Wilson^{2

3}, Siddarth Shivkumar^{4

5}, Hervé Rigneault⁴, Randy A Bartels^{1

6}

Affiliations

¹ Biomedical Imaging, Morgridge Institute for Research, Madison, Wisconsin 53715, United States.
² Department of Electrical Engineering, Colorado State University, Fort Collins, Colorado 80523, United States.
³ School of Biomedical Engineering, Colorado State University, Fort Collins, Colorado 80523, United States.
⁴ Aix Marseille University, CNRS, Centrale Med, Institut Fresnel, Marseille 13397, France.
⁵ Department of Physics, University of Ottawa, Ottawa, Ontario K1N6N5, Canada.
⁶ Department of Biomedical Engineering, University of Wisconsin, Madison, Wisconsin 53715, United States.

Abstract

We demonstrate low-frequency interferometric impulsive stimulated Raman scattering (ISRS) imaging with high robustness to distortions by optical scattering. ISRS is a pump-probe coherent Raman spectroscopy that can capture Raman vibrational spectra. Recording of ISRS spectra requires isolation of a probe pulse from the pump pulse. While this separation is simple in nonscattering specimens, such as liquids, scattering leads to significant pump pulse contamination and prevents the extraction of a Raman spectrum. We introduce a robust method for ISRS microscopy that works in complex scattering samples. High signal-to-noise ISRS spectra are obtained even when the pump and probe pulses pass through many scattering layers.

Grants and funding

R21 GM135772/GM/NIGMS NIH HHS/United States