Biogenic synthesis and characterization of silver nanoparticles (AgNPs) from aqueous extract of Lepidagathis cristata along with their antibacterial and antineoplastic activity to combat breast cancer cells (MCF-7)

Luminescence. 2024 Sep;39(9):e4891. doi: 10.1002/bio.4891.

Abstract

Lepidagathis cristata (L. cristata) plant produces reducing and capping agents; this study utilized microwave-assisted biogenic synthesis to manufacture silver nanoparticles (AgNPs) using this plant. The structure, morphology, and crystallinity phases of prepared nanoparticles (NPs) were characterized by ultraviolet-visible spectroscopy (UV-viz), powder X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, and scanning electron microscopy (SEM). Biologically synthesized AgNPs were treated against pathogenic bacteria species including Escherichia coli (E. coli), Bacillus subtilis (B. subtilis), and Staphylococcus aureus (S. aureus) and its highest zone of inhibition 10 ± 1.45 mm, 10 ± 0.74 mm, and 6 ± 0.43 mm, respectively, at the concentration of 100 μg/mL. The cytotoxic activity of AgNPs against MCF-7 breast cancer cells revealed significant growth inhibition by inhibiting cell viability, inhibitory concentration of 50% (IC50) of NPs observed at 55.76 μg/mL concentration. Finally, our findings concluded that the L. cristata-mediated biosynthesized AgNPs proved its potential antibacterial and neoplastic properties against MCF cells by endorsing the inhibition of cell proliferation especially with low concentration.

Keywords: L. cristata; antibacterial activity; antineoplastic; biogenic synthesis; silver nanoparticles.

MeSH terms

  • Anti-Bacterial Agents* / chemical synthesis
  • Anti-Bacterial Agents* / chemistry
  • Anti-Bacterial Agents* / pharmacology
  • Antineoplastic Agents / chemical synthesis
  • Antineoplastic Agents / chemistry
  • Antineoplastic Agents / pharmacology
  • Antineoplastic Agents, Phytogenic / chemical synthesis
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Bacillus subtilis / drug effects
  • Breast Neoplasms / drug therapy
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor*
  • Escherichia coli / drug effects
  • Female
  • Humans
  • MCF-7 Cells
  • Metal Nanoparticles* / chemistry
  • Microbial Sensitivity Tests*
  • Plant Extracts* / chemistry
  • Plant Extracts* / pharmacology
  • Silver* / chemistry
  • Silver* / pharmacology
  • Staphylococcus aureus / drug effects
  • Water / chemistry

Substances

  • Silver
  • Anti-Bacterial Agents
  • Plant Extracts
  • Antineoplastic Agents
  • Antineoplastic Agents, Phytogenic
  • Water