Highly selective separation and purification of lincomycin by macroporous adsorption resin column chromatography

Xue Yu; Houmao Chen; Zigan Sha; Yawen Hu; Mengxia Yan; Jianhui Xin; Xuejun Cao; Junfen Wan

doi:10.1016/j.chroma.2024.465282

Highly selective separation and purification of lincomycin by macroporous adsorption resin column chromatography

J Chromatogr A. 2024 Oct 25:1735:465282. doi: 10.1016/j.chroma.2024.465282. Epub 2024 Aug 17.

Authors

Xue Yu¹, Houmao Chen², Zigan Sha³, Yawen Hu², Mengxia Yan⁴, Jianhui Xin⁴, Xuejun Cao², Junfen Wan⁵

Affiliations

¹ State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, PR China; Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, PR China.
² State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, PR China.
³ Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, PR China.
⁴ Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou 253023, PR China.
⁵ State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, 130 Meilong Rd, Shanghai 200237, PR China. Electronic address: [email protected].

PMID: 39241407
DOI: 10.1016/j.chroma.2024.465282

Abstract

In this study, lincomycin was successfully purified by macroporous adsorption resin column chromatography using the HZ3 resin. The optimal separation parameters were set as follows: the column bed height was 33 cm, sample loading capacity was 48 mg/mL and flow rate of loading was 1 mL/min. A mixture of 0.02 mol/L of Na₂HPO₄∙12H₂O (pH = 8.5, adjusted using H₃PO₄) and acetone (80:20, v/v) was used as the eluent. The elution flow rate was maintained at 3 mL/min. Under these parameters, the purity of lincomycin calculated using the standard curve was 99.00 %, with the yield being 97.84 %. This enrichment and separation method of lincomycin is highly regarded owing to its remarkable efficiency and straightforward operation. Thus, the proposed method for the separation and purification of lincomycin holds considerable promise for pharmaceutical applications.

Keywords: Column chromatography; Lincomycin; Macroporous adsorption resin; Separation.

MeSH terms

Adsorption
Chromatography, High Pressure Liquid / methods
Lincomycin* / chemistry
Lincomycin* / isolation & purification
Porosity
Resins, Synthetic / chemistry

Substances

Lincomycin
Resins, Synthetic