Evaluation of the bioavailability of a Tamiflu taste-masking pediatric formulation using a juvenile pig model and LC-MS/MS

Bioanalysis. 2024;16(13):681-691. doi: 10.1080/17576180.2024.2352256. Epub 2024 Jun 11.

Abstract

Aim: To improve the palatability and increase compliance in pediatric patients, different taste-masking technologies have been evaluated to support the NIH Pediatric Formulation Initiative.Methods: This bioavailability approach combined a juvenile porcine model which represented the pediatric population, and an advanced UHPLCMS/MS method. Juvenile pigs were administered with either commercial Tamiflu or its taste-masking formulation and plasma samples were obtained from 0 to 48 h. The mass spectrometer was operated in positive mode with electrospray ionization.Results: The bioavailability profiles were not significantly different between the two formulations which demonstrated that taste-masking by forming an ionic complex was a promising approach for formulation modification.Conclusion: The pre-clinical study revealed a promising model platform for developing and screening taste-masking formulations.

Keywords: UHPLC-MS/MS; bioavailability; oseltamivir; pediatric; taste-masking formulation.

Plain language summary

[Box: see text].

MeSH terms

  • Animals
  • Biological Availability*
  • Child
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Liquid / methods
  • Humans
  • Liquid Chromatography-Mass Spectrometry
  • Oseltamivir* / administration & dosage
  • Oseltamivir* / blood
  • Oseltamivir* / pharmacokinetics
  • Swine
  • Tandem Mass Spectrometry* / methods
  • Taste*

Substances

  • Oseltamivir