Influence of Anticholinesterase Drugs on Activity and Properties of Na+,K+-ATPase in Rat Erythrocytes under Stress Caused by Intense Physical Exercise

Bull Exp Biol Med. 2024 Aug;177(4):427-430. doi: 10.1007/s10517-024-06202-z. Epub 2024 Sep 11.

Abstract

We studied the effect of intramuscular injection of physostigmine and neostigmine on Na+,K+-ATPase activity in erythrocytes of rats subjected to intense physical exercise. Both anticholinesterase drugs had a significant effect on the development of the stress response, which was expressed in a decrease in the manifestation of its individual components such as the concentration of ascorbic acid in the adrenal glands, stress-related erythrocyte polycythemia, and LPO indicators. Anticholinesterase drugs reverse the stress-induced decrease in Na+,K+-ATPase activity, as well as changes in its magnesium-dependent properties. There were no changes in the activity of the studied enzyme in the erythrocyte ghosts. We associate the observed differences with the correction of the functions of the cholinergic components of the hypothalamic-pituitary-adrenal axis leading to the development of a hypoergic type stress reaction.

Keywords: Na+,K+-ATPase; neostigmine; physostigmine; physical exercis.

MeSH terms

  • Adrenal Glands / drug effects
  • Adrenal Glands / enzymology
  • Adrenal Glands / metabolism
  • Animals
  • Ascorbic Acid / pharmacology
  • Cholinesterase Inhibitors* / pharmacology
  • Erythrocyte Membrane / drug effects
  • Erythrocyte Membrane / metabolism
  • Erythrocytes* / drug effects
  • Erythrocytes* / enzymology
  • Erythrocytes* / metabolism
  • Lipid Peroxidation / drug effects
  • Male
  • Neostigmine* / pharmacology
  • Physical Conditioning, Animal*
  • Physostigmine* / pharmacology
  • Rats
  • Rats, Wistar*
  • Sodium-Potassium-Exchanging ATPase* / metabolism
  • Stress, Physiological / drug effects

Substances

  • Sodium-Potassium-Exchanging ATPase
  • Cholinesterase Inhibitors
  • Physostigmine
  • Neostigmine
  • Ascorbic Acid