Nested PCR is a useful tool for identifying low-abundance target sequences of pathogens and avoiding false negatives. However, it carries an increased risk of cross-contamination, especially with its positive control. Here, we propose using customized synthetic oligonucleotides to detect false positives due to cross-contamination.
Keywords: Bartonella henselae; Contamination detection; False-positive; Molecular diagnostics; Nested PCR; Oligonucleotide.
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