Integrated, high-throughput metabolomics approach for metabolite analysis of four sprout types

Junyan Yu; Lei Xu; Lu Mi; Nan Zhang; Fengjuan Liu; Jing Zhao; Zhenzhen Xu

doi:10.1016/j.foodchem.2024.141182

Integrated, high-throughput metabolomics approach for metabolite analysis of four sprout types

Food Chem. 2025 Jan 15;463(Pt 2):141182. doi: 10.1016/j.foodchem.2024.141182. Epub 2024 Sep 6.

Authors

Junyan Yu¹, Lei Xu², Lu Mi³, Nan Zhang⁴, Fengjuan Liu⁵, Jing Zhao⁶, Zhenzhen Xu⁷

Affiliations

¹ Institute of Quality Standards and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing 100081, PR China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address: [email protected].
² Institute of Quality Standards and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing 100081, PR China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address: [email protected].
³ Institute of Quality Standards and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing 100081, PR China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address: [email protected].
⁴ Institute of Quality Standards and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing 100081, PR China. Electronic address: [email protected].
⁵ Institute of Quality Standards & Testing Technology for Agro-Products, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China. Electronic address: [email protected].
⁶ College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address: [email protected].
⁷ Institute of Quality Standards and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences, Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Beijing 100081, PR China; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, PR China. Electronic address: [email protected].

PMID: 39276547
DOI: 10.1016/j.foodchem.2024.141182

Abstract

In this study, we combined two distinct extraction and separation techniques with the aim of comprehensively collecting metabolite features in sprouts, particularly hydrophilic compounds. By synergistically analyzing the data using MS-DIAL and MetaboAnalystR, we obtained a greater number of annotated metabolites and explored differences in annotation across analytical tools. We found that this approach significantly increased the number of detected metabolite features and the final identification counts. Furthermore, we explored the functional component characteristics of four sprout types. This study provides data supporting the potential of sprouts as nutritious vegetables and functional food ingredients, emphasizing their value in the development of functional foods.

Keywords: Hydrophilic interaction chromatography technique; MS-DIAL; MetaboAnalystR; Metabolomics; Sprouts.

Publication types

Evaluation Study

MeSH terms

High-Throughput Screening Assays
Metabolomics*
Seedlings / chemistry
Seedlings / growth & development
Seedlings / metabolism
Vegetables / chemistry
Vegetables / metabolism