Novel report of Bipolaris heliconiae causing frog-eye-like leaf spot on Dypsis lutescens in Indian Sub-Continent

Microb Pathog. 2024 Nov:196:106938. doi: 10.1016/j.micpath.2024.106938. Epub 2024 Sep 12.

Abstract

Dypsis lutescens, commonly known as areca palm, is a highly valued ornamental species due to its aesthetic value. However, the foliage is vulnerable to various pathogens, particularly those responsible for fungal leaf spot diseases. In October 2023, a severe incidence (93 %) of destructive leaf spots was recorded on Dypsis lutescens at the University of Agricultural Sciences, GKVK, Bangalore, and surrounding areas. The leaf spot symptoms manifested as frog-eye-like lesions, leading to complete leaf desiccation and significantly reducing the palms ornamental value. The pathogen exhibited the highest radial growth (90.00 mm) and prominent sporulation on oat meal agar, whereas Richard's synthetic agar resulted in the lowest radial growth (38.00 mm) with no sporulation. Morphological and multilocus phylogenetic analyses confirmed the pathogen as Bipolaris heliconiae. Pathogenicity tests fulfilled Koch's postulates, confirming that Bipolaris heliconiae is the causative agent of leaf spot disease in Dypsis lutescens in India. This novel finding underscores the emergence of a new disease and highlights the urgent need for effective management strategies.

Keywords: Bipolaris heliconiae; Dypsis lutescens; First report; Multigene phylogeny.

MeSH terms

  • Arecaceae / microbiology
  • Ascomycota* / classification
  • Ascomycota* / genetics
  • Ascomycota* / growth & development
  • Ascomycota* / isolation & purification
  • Ascomycota* / pathogenicity
  • Ascomycota* / physiology
  • DNA, Fungal / genetics
  • India
  • Phylogeny*
  • Plant Diseases* / microbiology
  • Plant Leaves* / microbiology
  • Spores, Fungal / growth & development

Substances

  • DNA, Fungal