In-situ fabrication of resveratrol loaded sodium alginate coated silver nanoparticles for in vitro studies of mitochondrial-targeted anticancer treatment against MCF-7 cell lines

The study aims to improve the viability and stability of resveratrol by encapsulating metal-based biocompatible nanocarrier for mitochondrial-targeted delivery and breast cancer treatment. For this purpose, sodium alginate coated silver nanoparticles were synthesized by in-situ reduction of silver nitrate using sodium borohydride. The prepared nanoparticles and resveratrol-loaded nanoparticles were characterized by utilizing the following instruments including X-ray diffraction (XRD), UV visible spectroscopy, Photoluminescence (PL) spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray (EDX), Fourier Transform Infrared (FTIR), Raman spectroscopy, Zeta potential. The dialysis method revealed increased resveratrol release in pH 5 phosphate buffer. The incorporation of resveratrol significantly stimulated the antioxidant activity of sodium alginate coated silver nanoparticles. MTT assay was employed to evaluate the biocompatibility and anticancer potential of developed sodium alginate coated silver nanoparticles and resveratrol-loaded nanoparticles with increasing concentrations against normal HaCaT and breast cancer MCF-7 cell lines respectively. Further, the apoptotic morphology of MCF-7 cells treated with sodium alginate coated nanoparticles and resveratrol loaded nanoparticles was evaluated by AO/EtBr staining and apoptosis was demonstrated in the form of green and red fluorescence. Mitochondrial staining with Mito-Tracker Red evaluated the targeted delivery of RES into mitochondria leading to apoptosis of cancer cells.