Spectroscopic and chromatographic studies of chemical constituents of wild A.pindrow royle: facile foliar fuel for the synthesis of nanomaterials

Sadia Iram; Khuram Shahzad Ahmad; Irum Shaheen; Ahmed M Aljuwayid; Ghulam Abbas Ashraf

doi:10.1007/s12013-024-01500-9

Spectroscopic and chromatographic studies of chemical constituents of wild A.pindrow royle: facile foliar fuel for the synthesis of nanomaterials

Cell Biochem Biophys. 2024 Sep 23. doi: 10.1007/s12013-024-01500-9. Online ahead of print.

Authors

Sadia Iram¹, Khuram Shahzad Ahmad², Irum Shaheen³, Ahmed M Aljuwayid⁴, Ghulam Abbas Ashraf^{5

6}

Affiliations

¹ Department of Chemistry, Rawalpindi Women University, Rawalpindi, Pakistan.
² Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan. [email protected].
³ Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi, Pakistan.
⁴ Department of Chemistry, College of Science, King Saud University, P. O. Box 2455, Riyadh, 11451, Saudi Arabia.
⁵ College of Environment, Hohai University, Nanjing, 210098, China.
⁶ New Uzbekistan University, Mustaqillik Ave. 54, Tashkent, 100007, Uzbekistan.

PMID: 39312155
DOI: 10.1007/s12013-024-01500-9

Abstract

The current work is the first ever report on the functionalization of CoO nanoparticles (NPs) using the bio active constituents of Abies pindrow Royle (A.pindrow) leaves. An efficient phytochemical extraction method was determined by comparing different extraction strategies for extracting the biologically active compounds of A.pindrow leaves. The phytocompounds were noticed via chromatographic techniques; High-performance liquid chromatography (HPLC) as well as the Gas chromatography-mass spectroscopy (GC-MS) followed by spectroscopic analysis that is the Fourier transform infrared spectroscopy (FTIR) along with Ultraviolet-visible spectroscopy (UV-Vis). The reducing properties of the phytochemicals were investigated by efficiently synthesizing metal oxides nanoparticles (CoO NPs) by treating aqueous plant extract with Co(NO₃). 6H₂O aqueous complex. The newly synthesized NPs were characterized via X-ray diffraction (XRD), energy dispersive X-ray spectroscopy (EDX) and field emission-scanning electron microscopy (FE-SEM). Finally, the GCMS, FTIR and UV-Vis identified the A.pindrow leaves biocomponents as capping and reducing mediator of the synthesized CoO nanoparticles. FTIR confirmed the prepartion of CoO NPs as well as the capping and stabilizing agents of A.Pindrow at 2378.31 cm^-1, 1370.11 cm^-1, 1260.57 cm^-1, 937.4 cm^-1 and 607.24 cm^-1 having carboxylic acid, alcohols, aromatics, alkenes, aromatic amines, esters as well as ethers functional groups, flavonols and flavonoids phytochemicals. Moreover GCMS analysis revealed the dominating constituents of A.pindrow leaf extracts are carbohydrates, terpenoids, alkanoids, flavonoids as well as phenols. Furthermore, the antibacterial and bioactive agent, tannis was also observed in aqueous extract. These phytochemicals noticed in this current work, has antioxidant potential, that is why they have shown biomedical applications. The present manipulation, further articulated that, maximum phytochemicals extraction of A. pindrow leaves was illustrated in the aqueous extract as compared to ethyl acetate and ethanol.

Keywords: Bio-reducing agents; GC-MS; HPLC; Nanoparticles; Phyto-synthesis; Phytocapping agents; Phytochemicals screening.