Objectives: To observe the effect of ginger-salt-partitioned moxibustion on ATP-sensitive potassium (KATP) channel of bladder in detrusor overactivity (DO) rats.
Methods: Female SD rats were randomly divided into sham operation, model, moxibustion and antagonist groups (n=9 in each group). Thorax (T) 10 spinal cord transection was performed by surgery. Ginger-salt partitioned moxibustion was applied to "Shenque" (CV8) for 3 cones, once daily for 14 consecutive days. Rats of the antagonist group were intraperitoneally injected with KATP channel specific antagonist glibenclamide (10 μg·kg-1·d-1) once daily for 14 consecutive days. Urodynamic tests were performed after treatment. The distribution and expression of KATP channel tetrameric subunit (SUR2B) in the bladder of rats was observed by immunofluorescence. The protein and mRNA expression levels of SUR2B in bladder tissue were detected by Western blot and qPCR respectively.
Results: Compared with the sham operation group, rats of the model group showed intensive and large phasic contractions of the detrusor during bladder filling, the frequency and amplitude of phasic contractions of the detrusor 5 min before leakage were significantly increased (P<0.001)；the voiding threshold pressure was significantly decreased (P<0.001)；the bladder perfusion volume was increased (P<0.001)；the SUR2B protein and mRNA expression in bladder tissue were significantly reduced (P<0.001). Compared with the model group and the antagonist group, the above-mentioned indicators in the moxibustion group were all reversed (P<0.01, P<0.001, P<0.05).
Conclusions: Ginger-salt partitioned moxibustion can reduce the frequency and amplitude of detrusor phase contraction during bladder filling and prolong the time of first phase contraction in DO rats, which may be associated with up-regulating the expression level of KATP channel protein and mRNA, promoting the outflow of potassium ions, and inhibiting the inflow of calcium ions, thus improve the stability of detrusor during storage.
目的: 观察隔姜隔盐灸“神阙”对逼尿肌过度活动（DO）大鼠膀胱ATP敏感性钾离子（KATP）通道的影响，探讨灸法治疗DO的部分机制。方法: SD雌性大鼠采用胸（T）10完全脊髓横断术建立DO大鼠模型，纳入成模大鼠27只，随机分为模型组、隔姜隔盐灸组、拮抗组，每组9只，另取9只大鼠作为假手术组。隔姜隔盐灸组予隔姜隔盐灸“神阙”，拮抗组每日腹腔注射KATP通道特异性拮抗剂格列本脲后予隔姜隔盐灸“神阙”，两组均每次连灸3壮，每日1次，连续治疗14 d。治疗后进行尿流动力学检测；免疫荧光染色法观察KATP通道四聚体亚基（SUR2B）在大鼠膀胱中的分布及表达，采用Western blot、实时荧光定量PCR法检测膀胱组织SUR2B蛋白及mRNA表达水平。结果: 与假手术组相比，模型组大鼠在膀胱充盈期间出现密集且大幅度的逼尿肌期相性收缩，漏尿前5 min逼尿肌期相性收缩发生频率及幅度均升高（P<0.001），膀胱灌注量升高（P<0.001），排尿阈值压降低（P<0.001）；膀胱组织中SUR2B的表达、蛋白及mRNA相对表达量均降低（P<0.001）。与模型组及拮抗组比较，隔姜隔盐灸组上述指标均逆转（P<0.01，P<0.001，P<0.05）。结论: 隔盐隔姜灸“神阙”能够降低DO大鼠膀胱充盈期间逼尿肌期相性收缩的频率及幅度，延长首次期相性收缩发生时间，其机制可能为上调DO大鼠膀胱组织中KATP通道蛋白及mRNA表达水平，进而促进钾离子的外流，抑制钙离子的内流，提高储尿期逼尿肌的稳定性。.
Keywords: Detrusor overactivity; K ATP channel; Moxibustion.