Development of nr-NDV.Reverse transfection was applied for the recovery of nr-NDV.Propagation of nr-NDV was done by sub-passaging transfected BSR T7/5 cells.Safety profile was done to prove that the nr-NDV is non-replicating.
Keywords: Newcastle disease virus; avian virus; non-replicating; reverse genetic system; truncated anti-genome; viral vector.