Standardization and quality assessment for human intestinal organoids

Hana Lee; Seunghye Yang; Kyung Jin Lee; Si-Na Kim; Ji-Seon Jeong; Ki Young Kim; Cho-Rok Jung; Sooyeon Jeon; Dayeon Kwon; Sungin Lee; Hanbyeol Lee; Chihye Park; Sun-Ju Ahn; Jongman Yoo; Mi-Young Son

doi:10.3389/fcell.2024.1383893

Standardization and quality assessment for human intestinal organoids

Front Cell Dev Biol. 2024 Sep 12:12:1383893. doi: 10.3389/fcell.2024.1383893. eCollection 2024.

Authors

Hana Lee^#^{1

2}, Seunghye Yang^#^{2

3}, Kyung Jin Lee^#^{2

3}, Si-Na Kim³, Ji-Seon Jeong^{2

4}, Ki Young Kim^{2

5}, Cho-Rok Jung^{1

2

6}, Sooyeon Jeon⁷, Dayeon Kwon⁷, Sungin Lee⁷, Hanbyeol Lee⁷, Chihye Park⁷, Sun-Ju Ahn^{2

7}, Jongman Yoo^{2

3

8}, Mi-Young Son^{1

2

6

9}

Affiliations

¹ Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
² Organoid Standards Initiative (OSI), Department of Biophysics, Institute of Quantum Biophysics, Sungkyunkwan University, Suwon, Republic of Korea.
³ ORGANOIDSCIENCES, Seongnam-si, Republic of Korea.
⁴ Korea Research Institute of Standards and Science (KRISS), Daejeon, Republic of Korea.
⁵ Therapeutics and Biotechnology Division, Korea Research Institute of Chemical Technology, Daejeon, Republic of Korea.
⁶ KRIBB School of Bioscience, Korea University of Science and Technology (UST), Daejeon, Republic of Korea.
⁷ Digital Health Laboratory, Department of Biophysics, Sungkyunkwan University, Suwon, Republic of Korea.
⁸ Department of Microbiology, CHA University School of Medicine, Seongnam-si, Republic of Korea.
⁹ Department of Biological Science, Sungkyunkwan University, Suwon, Republic of Korea.

^# Contributed equally.

Abstract

To enhance the practical application of intestinal organoids, it is imperative to establish standardized guidelines. This proposed standardization outlines a comprehensive framework to ensure consistency and reliability in the development, characterization, and application of intestinal organoids. The recommended guidelines encompass crucial parameters, including culture conditions, critical quality attributes, quality control measures, and functional assessments, aimed at fostering a standardized approach across diverse research initiatives. The implementation of these guidelines is anticipated to significantly contribute to the reproducibility and comparability of results in the burgeoning field of intestinal organoid research.

Keywords: guideline; human adult stem cell (hASC); human intestinal organoid (hIO); human pluripotent stem cell (hPSC); standardization.

Grants and funding

The authors declare that financial support was received for the research, authorship, and/or publication of this article. This work was supported by a grant from the Korean Fund for Regenerative Medicine (KFRM) grant funded by the Korean government (Ministry of Science and ICT, Ministry of Health and Welfare, 21A0404L1), the grant (23212MFDS265) from Ministry of Food and Drug Safety in 2023, a grant (22213MFDS386) from Ministry of Food and Drug Safety in 2024, a grant of a grant (23192MFDS097) from Ministry of Food and Drug Safety in 2023, a grant from the Technology Innovation Program (20018578, Production standardization and development of analysis to verify the quality and characterization of organoid based regeneration medicine) funded By the Ministry of Trade, Industry and Energy (MOTIE, Korea), a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, Republic of Korea (grant number: HI22C1462) and the KRIBB Research Initiative Program (KGM4722432).