In early 2024, explosive outbreaks of Oropouche virus (OROV) linked to a novel lineage were documented in the Amazon Region of Brazil. We report the introduction of this lineage into Colombia and its co-circulation with another OROV lineage. Continued surveillance is needed to prevent further spread of OROV in the Americas.
Keywords: Amazon Basin; Colombia; Leticia; Oropouche virus; fever; lineages; outbreak; reassortant; reassortment; sequencing; vector-borne infections; viruses; zoonoses.