Influence of fractionation of polyphenols by membrane chromatography on antioxidant, antimicrobial and proliferation-inhibiting effects of red fruit juices

Food Chem. 2025 Jan 15;463(Pt 2):141216. doi: 10.1016/j.foodchem.2024.141216. Epub 2024 Sep 12.

Abstract

Interest in fruit juice extracts as nutraceuticals is constantly increasing due to their health-beneficial properties, mainly caused by polyphenols. However, the correlation between the various effects of fruit juice extracts and their individual composition, including anthocyanins and copigments, is unknown. Therefore, in the present study, eight red fruit juice extracts were prepared using XAD-7 column chromatography, followed by fractionation and identification of the different compounds as well as characterization of their health-promoting effects. The fruit juice extract of pomegranate, chokeberry, and cranberry showed the highest antimicrobial potential against food-borne pathogens. The highest antioxidant and cell proliferation-inhibiting potential was also found in the pomegranate extract. It can be assumed that pomegranate extracts, which are rich in copigments, especially hydrolyzable tannins, are suitable natural antioxidants and antimicrobial agents. Pomegranate extracts could be used as nutraceuticals or natural preservatives.

Keywords: Anthocyanin; Berry; Bioactivity; Cell culture; Flavonoid; HPLC.

MeSH terms

  • Anti-Infective Agents* / chemistry
  • Anti-Infective Agents* / pharmacology
  • Antioxidants* / chemistry
  • Antioxidants* / isolation & purification
  • Antioxidants* / pharmacology
  • Bacteria / drug effects
  • Cell Proliferation / drug effects
  • Chemical Fractionation
  • Chromatography
  • Fruit / chemistry
  • Fruit and Vegetable Juices* / analysis
  • Growth Inhibitors / chemistry
  • Growth Inhibitors / pharmacology
  • Humans
  • Plant Extracts* / chemistry
  • Plant Extracts* / isolation & purification
  • Plant Extracts* / pharmacology
  • Polyphenols* / chemistry
  • Polyphenols* / isolation & purification
  • Polyphenols* / pharmacology
  • Pomegranate* / chemistry
  • Vaccinium macrocarpon / chemistry

Substances

  • Polyphenols
  • Antioxidants
  • Anti-Infective Agents
  • Plant Extracts
  • Growth Inhibitors