LAPONITE® nano-silicates potentiate the angiogenic effects of FG-4592 and osteogenic effects of BMP-2

Biomater Sci. 2024 Oct 22;12(21):5610-5619. doi: 10.1039/d4bm00636d.

Abstract

LAPONITE®-based drug delivery systems offer many advantages due to the unique ionic and physical properties of LAPONITE®. The high ionicity and large surface area of LAPONITE® nanoparticles enable the intercalation and dissolution of biomolecules. In this study, we explored the potential of LAPONITE® as a carrier for FG-4592 to support angiogenesis and as a carrier for bone morphogenic protein-2 (BMP-2) to support osteogenesis. Interestingly, we found that LAPONITE® promoted the FG-4592 induced upregulation of vascular endothelial growth factor (VEGF) gene expression of human umbilical cord endothelial cells (HUVECs). Additionally, we observed that LAPONITE® could provide a sustained release of BMP-2 and significantly potentiate the osteogenic effects of BMP-2 on adipose derived mesenchymal stem cells (AMSCs). Overall, current findings on the LAPONITE®-drug/protein model system provide a unique way to potentiate the angiogenic activities of FG-4592 on HUVECs and osteogenic effects of BMP-2 on AMSCs for tissue engineering application. Future studies will be directed towards gaining a deeper understanding of these effects on a co-culture system of HUVECs and AMSCs.

MeSH terms

  • Bone Morphogenetic Protein 2* / metabolism
  • Bone Morphogenetic Protein 2* / pharmacology
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Human Umbilical Vein Endothelial Cells / drug effects
  • Humans
  • Mesenchymal Stem Cells* / drug effects
  • Nanoparticles / chemistry
  • Neovascularization, Physiologic* / drug effects
  • Osteogenesis* / drug effects
  • Silicates* / chemistry
  • Silicates* / pharmacology
  • Vascular Endothelial Growth Factor A / metabolism
  • Vascular Endothelial Growth Factor A / pharmacology

Substances

  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • laponite
  • Silicates
  • Vascular Endothelial Growth Factor A