Psilocybin reduces functional correlation and the encoding of spatial information by neurons in mouse retrosplenial cortex

Eur J Neurosci. 2024 Nov;60(10):6395-6407. doi: 10.1111/ejn.16558. Epub 2024 Oct 4.

Abstract

Psychedelic drugs have profound effects on perception, cognition and mood. How psychedelics affect neural signaling to produce these effects remains poorly understood. We investigated the effect of the classic psychedelic psilocybin on neural activity patterns and spatial encoding in the retrosplenial cortex of head-fixed mice navigating on a treadmill. The place specificity of neurons to distinct locations along the belt was reduced by psilocybin. Moreover, the stability of place-related activity across trials decreased. Psilocybin also reduced the functional correlation among simultaneously recorded neurons. The 5-HT2AR (serotonin 2A receptor) antagonist ketanserin blocked these effects. These data are consistent with proposals that psychedelics increase the entropy of neural signaling and provide a potential neural mechanism contributing to disorientation frequently reported by humans after taking psychedelics.

Keywords: ketanserin; mutual information; psychedelics; retrosplenial cortex; serotonin.

MeSH terms

  • Animals
  • Gyrus Cinguli / drug effects
  • Gyrus Cinguli / physiology
  • Hallucinogens* / pharmacology
  • Ketanserin / pharmacology
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neurons* / drug effects
  • Neurons* / physiology
  • Psilocybin* / pharmacology
  • Serotonin 5-HT2 Receptor Antagonists / pharmacology
  • Space Perception / drug effects
  • Space Perception / physiology

Substances

  • Psilocybin
  • Hallucinogens
  • Ketanserin
  • Serotonin 5-HT2 Receptor Antagonists