Recent advances in correlative cryo-light and electron microscopy

Joshua A Pierson; Jie E Yang; Elizabeth R Wright

doi:10.1016/j.sbi.2024.102934

Recent advances in correlative cryo-light and electron microscopy

Curr Opin Struct Biol. 2024 Dec:89:102934. doi: 10.1016/j.sbi.2024.102934. Epub 2024 Oct 3.

Authors

Joshua A Pierson¹, Jie E Yang², Elizabeth R Wright³

Affiliations

¹ Department of Biochemistry, University of Wisconsin, Madison, WI, USA.
² Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA.
³ Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Cryo-Electron Microscopy Research Center, Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Midwest Center for Cryo-Electron Tomography, Department of Biochemistry, University of Wisconsin, Madison, WI, USA; Morgridge Institute for Research, Madison, WI, USA. Electronic address: [email protected].

PMID: 39366119
PMCID: PMC11602379 (available on 2025-12-01)
DOI: 10.1016/j.sbi.2024.102934

Abstract

Correlative light and electron microscopy (CLEM) pipelines serve to integrate the imaging modalities of fluorescence light microscopy (FLM) and cryogenic electron microscopy (cryo-EM) to produce contextually relevant high-resolution structural snapshots of biological systems. Innovations in sample preparation, instrumentation, imaging, and data processing have advanced the field of cryo-EM. This review focuses on prior work and recent developments in the field of cryo- EM that support further integration of technologies for correlative microscopy workflows.

Publication types

Review

MeSH terms

Animals
Cryoelectron Microscopy* / methods
Humans
Image Processing, Computer-Assisted / methods
Microscopy, Fluorescence / methods

Abstract

Publication types

MeSH terms

Grants and funding