Abstract
Three previously undescribed coumarins (1-3) were obtained from the roots of Notopterygium incisum. Their chemical structures were elucidated using a variety of spectroscopic techniques and chemical calculations. The inhibitory effects of these new compounds on NO production and pro-inflammatory factors (IL-1β, IL-6, and TNF-α) in LPS-stimulated RAW 264.7 cells were investigated. Further studies revealed that compound 1 suppressed the expression of COX-2 and iNOS while also reduced ROS accumulation. Western blot analysis demonstrated that compound 1 could inhibit the PI3K/AKT pathway by decreasing the levels of p-PI3K and p-AKT. Collectively, these findings suggest that compounds 1-3 exhibit promising anti-inflammatory properties.
Keywords:
Notopterygium incisum; PI3K/AKT pathway; Umbelliferae; anti-inflammatory activities; coumarins.
MeSH terms
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Animals
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Anti-Inflammatory Agents* / chemistry
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Anti-Inflammatory Agents* / pharmacology
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Apiaceae* / chemistry
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Coumarins* / chemistry
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Coumarins* / isolation & purification
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Coumarins* / pharmacology
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Cyclooxygenase 2 / metabolism
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Interleukin-1beta / metabolism
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Interleukin-6 / metabolism
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Lipopolysaccharides* / pharmacology
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Mice
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Molecular Structure
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Nitric Oxide Synthase Type II / antagonists & inhibitors
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Nitric Oxide* / antagonists & inhibitors
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Nitric Oxide* / biosynthesis
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Phosphatidylinositol 3-Kinases / metabolism
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Plant Roots / chemistry
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Proto-Oncogene Proteins c-akt / metabolism
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RAW 264.7 Cells
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Reactive Oxygen Species / metabolism
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Tumor Necrosis Factor-alpha*
Substances
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Coumarins
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Anti-Inflammatory Agents
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Lipopolysaccharides
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Nitric Oxide
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Tumor Necrosis Factor-alpha
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Interleukin-6
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Nitric Oxide Synthase Type II
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Cyclooxygenase 2
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Interleukin-1beta
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Phosphatidylinositol 3-Kinases
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Reactive Oxygen Species
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Proto-Oncogene Proteins c-akt