A HOF-101@AgNPs-based dual-signal mode aptasensor for electrochemiluminescence and fluorescence detection of E. coli O157:H7

Food Chem. 2025 Feb 1;464(Pt 1):141591. doi: 10.1016/j.foodchem.2024.141591. Epub 2024 Oct 9.

Abstract

Escherichia coli O157:H7 (E. coli O157:H7) emerges as a foodborne pathogen, emphasizing the imperative for creating precise detection tools. An ultrasensitive electrochemiluminescence/fluorescence (ECL/FL) dual-signal mode aptasensor was constructed for the detection of E. coli O157:H7. Ultrafine silver nanoparticles were loaded on the surface of hydrogen-bonded organic skeleton materials by in-situ photoreduction method, and then combined with aptamers that can identify specific targets to prepare HOF-101@AgNPs@Apt, greatly simplifying the ECL/FL dual-signal mode probe fabrication process and improving sensing reliability. HOF-101@AgNPs@Apt had both strong ECL luminescence and fluorescence, resulting in a high detection sensitivity. The low limit of detection (LOD) for ECL and FL were 0.48 CFU mL-1 and 2.39 CFU mL-1, respectively. Moreover, the proposed dual-signal mode aptasensor has been successfully applied to determine E. coli O157:H7 levels in tap water and milk with superior accuracy and high antiinterference capability, providing a promising method for food safety monitoring.

Keywords: Aptasensor; Dual-signal mode; Electrochemiluminescence; Escherichia coli O157:H7; Fluorescence.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Aptamers, Nucleotide* / chemistry
  • Biosensing Techniques* / instrumentation
  • Electrochemical Techniques* / instrumentation
  • Escherichia coli O157* / isolation & purification
  • Fluorescence
  • Food Contamination / analysis
  • Limit of Detection
  • Luminescent Measurements* / instrumentation
  • Luminescent Measurements* / methods
  • Metal Nanoparticles* / chemistry
  • Milk* / chemistry
  • Milk* / microbiology
  • Silver* / chemistry

Substances

  • Aptamers, Nucleotide
  • Silver