A multifunctional oxidase-mimicking Ag/Mn3O4 was prepared, catalyzing the 3, 3', 5, 5'-tetramethylbenzidine (TMB) chromogenic reaction. Six foodborne pathogenic bacteria species, including Escherichia coli, Staphylococcus aureus, Salmonella enterica, Listeria monocytogenes, Bacillus cereus, and Cronobacter sakazakii, were observed to differentially inhibit its oxidase-like activity, resulting in decelerating the TMB chromogenic reaction. Owing to these properties, the following achievements were achieved: colorimetric determination of these bacteria with high sensitivity can be achieved using Ag/Mn3O4 + TMB reaction system; precise identification of these bacteria at different concentrations, including individual bacterium, binary mixtures, and even multivariate mixtures, can be effectively realized by combining the Ag/Mn3O4-based colorimetric sensor array with principal component analysis (PCA); broad-spectrum inactivation of these bacteria can be remarkably realized through catalyzation of Ag/Mn3O4 to generate superoxide anion free radicals. Therefore, our proposed Ag/Mn3O4 holds significant application potential in the colorimetric determination, precise identification, and broad-spectrum inactivation of foodborne pathogenic bacteria.
Keywords: Broad-spectrum inactivation; Colorimetric determination; Foodborne pathogenic bacteria; Multifunctional Ag/Mn(3)O(4) nanozyme; Oxidase-mimicking activity; Precise identification.
Copyright © 2024 Elsevier Ltd. All rights reserved.