Establishing a quantitative detection method for adulterated meat products is crucial for ensuring food safety. However, due to the lack of metrological traceability in current quantitative methods, poor reliability and comparability always occur when measuring adulterated meat. Therefore, this study established an accurate quantitative method with metrological traceability for the quantitative analysis of pork and duck meat added to beef, lamb, and their products. We took the amino acid certified reference materials (CRMs) as the source of traceability, and traced the quantitative peptide concentrations of adulterated beef, lamb and their products to SI. Finally, the quantitative method has high accuracy and high repeatability and we utilized it to achieve accurate quantification of adulterated meat products, with an uncertainty range of 10.7 %-18.5 %, k = 2.
Keywords: Isotope dilution mass spectrometry; LC-MS/MS; Meat adulteration; Metrological traceability; Proteomics.
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