Survival of antimicrobial resistant Salmonella Heidelberg inoculated into microcosms of fresh pine wood shavings for broiler litter

Adelumola Oladeinde; Kimberly Cook; Attiq Rehman; Catherine D Carrillo; Reed Woyda; Crystal Wiersma; Zaid Abdo; Jasmine Johnson; Anna Marie Bosch; Michael Rothrock Jnr; Moussa S Diarra

doi:10.1139/cjm-2024-0088

Survival of antimicrobial resistant Salmonella Heidelberg inoculated into microcosms of fresh pine wood shavings for broiler litter

Can J Microbiol. 2024 Oct 22. doi: 10.1139/cjm-2024-0088. Online ahead of print.

Authors

Adelumola Oladeinde¹, Kimberly Cook¹, Attiq Rehman², Catherine D Carrillo³, Reed Woyda^{4

5}, Crystal Wiersma^{4

5}, Zaid Abdo^{4

5}, Jasmine Johnson⁶, Anna Marie Bosch⁷, Michael Rothrock Jnr¹, Moussa S Diarra²

Affiliations

¹ U.S. National Poultry Research Center, USDA-ARS, Athens, GA, USA.
² Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, Canada.
³ Canadian Food Inspection Agency, Ottawa Laboratory (Carling), Ottawa, ON, Canada.
⁴ Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA.
⁵ Program of Cell and Molecular Biology, Colorado State University, Fort Collins, CO, USA.
⁶ Department of Poultry Science, University of Georgia, Athens, GA, USA.
⁷ College of Public Health, University of Georgia, Athens, GA, USA.

PMID: 39437442
DOI: 10.1139/cjm-2024-0088

Abstract

S. Heidelberg survived up to 21 days in PWS which is often used as broiler bedding. S. Heidelberg abundance and survival was correlated with the water activity of PWS. S. Heidelberg strains that carried higher copy numbers of small Col plasmids were the dominant strains isolated from PWS at later time points. S. Heidelberg strains harboring transmissible plasmid carrying AmpC-like beta-lactamase gene persisted longer in PWS without antibiotic pressures for AMR.

Keywords: AMR; Salmonella; broiler litter; pine wood shavings (PWS); plasmids.