Lack of Candida africana in Ugandan pregnant women: results from a pilot study using MALDI-ToF

BMC Res Notes. 2024 Oct 24;17(1):321. doi: 10.1186/s13104-024-06973-8.

Abstract

Background: Candida africana is an emergent variant that has been listed as a new species or variety within the Candida albicans complex since 2001. It has a worldwide intra-albicans complex pooled prevalence of 1.67% and varies between 0 and 8% depending on geographical region. We present the results of a pilot study on its prevalence in Uganda.

Methodology: We conducted a cross-sectional study between March and June 2023. We recruited 4 pregnant women from Mulago Specialized Women and Neonatal Hospital, 102 from Kawempe National Referral Hospital, and 48 from Sebbi Hospital. Vaginal swabs were tested using microscopy, culture and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF).

Results: The prevalence of C. africana was zero. Out of the 103 isolates, the majority (81.553%) were identified as Candida albicans, followed by Nakeseomyces glabrata (13.592%) and Pichia kudriavzevii (1.942%). Cyberlindnera jadinii, Candida tropicalis, and Candida parapsilosis each accounted for 0.971% of the isolates.

Conclusion: The prevalence of C. africana in Uganda is zero. However, large-scale cross-sectional studies, including studies involving the collection of vaginal samples from both urban and rural settings in Uganda and the use of both MALDI-TOF- and PCR-based laboratory methods, are needed to fully describe the public health burden of C. africana infections.

Keywords: C. africana; C. albicans; C. glabrata; C. parapsilosis; C. tropicalis; Cyberlindnera jadinii; Pichia kudriavzevii; Uganda.

MeSH terms

  • Adult
  • Candida albicans / isolation & purification
  • Candida tropicalis / isolation & purification
  • Candida* / classification
  • Candida* / isolation & purification
  • Cross-Sectional Studies
  • Female
  • Humans
  • Pichia
  • Pilot Projects
  • Pregnancy
  • Prevalence
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / methods
  • Uganda / epidemiology
  • Vagina / microbiology
  • Young Adult

Supplementary concepts

  • Pichia kudriavzevii