Electro-stimulated biodegradation of dimethyl disulfide: Insights from biofilm spatial structure and key functional genes

As a typical sulfur-containing volatile organic compound, dimethyl disulfide (DMDS) is known for its high toxicity and resistance to degradation, necessitating efficient control in environmental media. To address the limitations of biological treatment in degradation capacity, this study employs electro-stimulation to promote DMDS elimination by a porous polyaniline@carbon nanotube bioanode developed on graphite sheet (PANI@CNT/GS). Compared with the unmodified GS bioanode, the PANI@CNT/GS bioanode demonstrates significant advantages in biofilm activity, redox property, and DMDS degradation efficiency. Kinetics analysis shows that the maximum degradation rate of the PANI@CNT/GS bioanode was 0.60 mM h^-1, which is 1.36 times higher than that of the control. Characterization results reveal that the highly active biofilms in PANI@CNT/GS bioanode possess 1.40 times the amount of living cells and a 12.5% increase in thickness, contributing to the notable enhancement in DMDS degradation capacity. Additionally, functional gene annotation indicates that the PANI@CNT/GS electrode facilitates the motility and activity of microbial cells and enriches the genes encoding key enzymes involved in DMDS metabolism. This work validates the feasibility of electro-stimulation for enhancing DMDS degradation and further provides in-depth insights into the process intensification mechanism from the perspectives of biofilm spatial structure and key functional genes.