Single-Cell Analysis of Bone Marrow CD8+ T Cells in Myeloid Neoplasms Reveals Pathways Associated with Disease Progression and Response to Treatment with Azacitidine

Athanasios Tasis; Nikos E Papaioannou; Maria Grigoriou; Nikolaos Paschalidis; Catherine Loukogiannaki; Anastasia Filia; Kyriaki Katsiki; Eleftheria Lamprianidou; Vasileios Papadopoulos; Christina Maria Rimpa; Antonios Chatzigeorgiou; Ioannis Kourtzelis; Petroula Gerasimou; Ioannis Kyprianou; Paul Costeas; Panagiotis Liakopoulos; Konstantinos Liapis; Petros Kolovos; Triantafyllos Chavakis; Themis Alissafi; Ioannis Kotsianidis; Ioannis Mitroulis

doi:10.1158/2767-9764.CRC-24-0310

Single-Cell Analysis of Bone Marrow CD8+ T Cells in Myeloid Neoplasms Reveals Pathways Associated with Disease Progression and Response to Treatment with Azacitidine

Cancer Res Commun. 2024 Dec 1;4(12):3067-3083. doi: 10.1158/2767-9764.CRC-24-0310.

Authors

Athanasios Tasis^{1

2}, Nikos E Papaioannou^#³, Maria Grigoriou^#^{1

4}, Nikolaos Paschalidis^#⁴, Catherine Loukogiannaki⁴, Anastasia Filia^{1

4}, Kyriaki Katsiki¹, Eleftheria Lamprianidou², Vasileios Papadopoulos², Christina Maria Rimpa^{1

2}, Antonios Chatzigeorgiou⁵, Ioannis Kourtzelis⁶, Petroula Gerasimou⁷, Ioannis Kyprianou⁷, Paul Costeas⁷, Panagiotis Liakopoulos⁸, Konstantinos Liapis², Petros Kolovos⁸, Triantafyllos Chavakis^{9

10}, Themis Alissafi^{3

11}, Ioannis Kotsianidis^#², Ioannis Mitroulis^#^{1

2

9

10}

Affiliations

¹ Translational Research and Laboratory Medicine Unit, First Department of Internal Medicine, University Hospital of Alexandroupolis, Democritus University of Thrace, Alexandroupolis, Greece.
² Department of Hematology, University Hospital of Alexandroupolis, Democritus University of Thrace, Alexandroupolis, Greece.
³ Laboratory of Immune Regulation, Center of Basic Sciences, Biomedical Research Foundation Academy of Athens, Athens, Greece.
⁴ Laboratory of Autoimmunity and Inflammation, Center of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation Academy of Athens, Athens, Greece.
⁵ Department of Physiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
⁶ Hull York Medical School, York Biomedical Research Institute, University of York, York, United Kingdom.
⁷ Molecular Hematology-Oncology, Karaiskakio Foundation, Nicosia, Cyprus.
⁸ Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.
⁹ Institute for Clinical Chemistry and Laboratory Medicine, University Hospital and Faculty of Medicine Carl Gustav Carus of TU Dresden, Dresden, Germany.
¹⁰ National Center for Tumor Diseases, Partner Site Dresden, Dresden, Germany.
¹¹ Laboratory of Biology, School of Medicine, Athens, Greece.

^# Contributed equally.

Abstract

Immunophenotypic analysis identified a BM CD57+CXCR3+ subset of CD8+ T cells associated with response to AZA in patients with MDS and AML. Single-cell RNA sequencing analysis revealed that IFN signaling is linked to the response to treatment, whereas TGF-β signaling is associated with treatment failure, providing insights into new therapeutic approaches.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aged
Antimetabolites, Antineoplastic / pharmacology
Antimetabolites, Antineoplastic / therapeutic use
Azacitidine* / pharmacology
Azacitidine* / therapeutic use
Bone Marrow / drug effects
Bone Marrow / pathology
CD8-Positive T-Lymphocytes* / drug effects
CD8-Positive T-Lymphocytes* / immunology
Disease Progression*
Female
Humans
Leukemia, Myeloid, Acute / drug therapy
Leukemia, Myeloid, Acute / immunology
Leukemia, Myeloid, Acute / pathology
Male
Middle Aged
Myelodysplastic Syndromes* / drug therapy
Myelodysplastic Syndromes* / pathology
Signal Transduction / drug effects
Single-Cell Analysis*

Substances

Azacitidine
Antimetabolites, Antineoplastic