Early diagnosis of immunodeficient patients with partial albinism: The role of hair study and peripheral blood smear

Pediatr Allergy Immunol. 2024 Nov;35(11):e14264. doi: 10.1111/pai.14264.

Abstract

Background: Primary immunodeficiency diseases (inborn errors of immunity) with partial albinism are a group of autosomal recessive syndromes including Chediak Higashi Syndrome (CHS), Griscelli Syndrome type 2 (GS2), Hermansky-Pudlak Syndromes type 2 and 10 (HPS2, HPS10), Vici syndrome and P14/LAMTOR2 deficiency.

Methods: Twenty-five patients including 10 CHS, 10 GS2, and 5 HPS2 were evaluated in this study within the last 10 years. Five cases with oculocutaneous albinism (OCA) and 5 healthy subjects without albinism were used as two control groups. Genetic analyses were performed by whole exome or panel sequencing or targeted Sanger sequencing. Subsequently, leukocyte granules in peripheral blood smear and hair shaft were examined as screening tests.

Results: Giant granules were only presented in the leukocytes cytoplasm of 10/10 CHS patients. The uneven cluster of pigments and giant melanin granules in hair samples were observed in 10/10 GS2 and 10/10 CHS patients, respectively. In both 5/5 OCA and 5/5 HPS2 patients, there were regular pigments in the middle of hair shafts. Genetic analyses were performed for all patients, revealing 7 novel variants in LYST gene for CHS patients and 4 novel variants in AP3B1 for HPS2 patients.

Conclusion: Receiving hematopoietic stem cell transplantation (HSCT) in a timely manner is crucial in CHS and GS2 patients; therefore, screening tests may provide a vital clue for early diagnosis in these patients. However, the final confirmation of CHS, GS2, and HPS2 disorders is done by genetic assay.

Keywords: Chediak higashi syndrome; Griscelli syndrome type 2; Hermanskey pudlak syndrome type 2; novel causal variants; partial albinism; primary immunodeficiency diseases.

MeSH terms

  • Adolescent
  • Albinism / diagnosis
  • Albinism / genetics
  • Albinism, Oculocutaneous / diagnosis
  • Albinism, Oculocutaneous / genetics
  • Chediak-Higashi Syndrome / diagnosis
  • Chediak-Higashi Syndrome / genetics
  • Child
  • Child, Preschool
  • Early Diagnosis*
  • Female
  • Genetic Testing / methods
  • Hair* / immunology
  • Hermanski-Pudlak Syndrome / diagnosis
  • Hermanski-Pudlak Syndrome / genetics
  • Humans
  • Immunologic Deficiency Syndromes / diagnosis
  • Immunologic Deficiency Syndromes / genetics
  • Immunologic Deficiency Syndromes / immunology
  • Infant
  • Lymphohistiocytosis, Hemophagocytic
  • Male
  • Mutation
  • Piebaldism / diagnosis
  • Piebaldism / genetics
  • Primary Immunodeficiency Diseases / diagnosis
  • Primary Immunodeficiency Diseases / genetics
  • Primary Immunodeficiency Diseases / immunology

Supplementary concepts

  • Griscelli syndrome type 2