Revolutionizing animal husbandry: Breakthroughs in gene editing delivery systems

Yuan Liu; Xue Bai; Xue Feng; Shuang Liu; Yamei Hu; Hongen Chu; Lingkai Zhang; Bei Cai; Yun Ma

doi:10.1016/j.gene.2024.149044

Revolutionizing animal husbandry: Breakthroughs in gene editing delivery systems

Gene. 2025 Jan 30:935:149044. doi: 10.1016/j.gene.2024.149044. Epub 2024 Oct 28.

Authors

Yuan Liu¹, Xue Bai², Xue Feng³, Shuang Liu⁴, Yamei Hu⁵, Hongen Chu⁶, Lingkai Zhang⁷, Bei Cai⁸, Yun Ma⁹

Affiliations

¹ College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, Ningxia University, Yinchuan 750021, China. Electronic address: [email protected].
² College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, Ningxia University, Yinchuan 750021, China.
³ College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, Ningxia University, Yinchuan 750021, China. Electronic address: [email protected].
⁴ College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, Ningxia University, Yinchuan 750021, China. Electronic address: [email protected].
⁵ College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, Ningxia University, Yinchuan 750021, China. Electronic address: [email protected].
⁶ College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, Ningxia University, Yinchuan 750021, China. Electronic address: [email protected].
⁷ College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, Ningxia University, Yinchuan 750021, China. Electronic address: [email protected].
⁸ College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, Ningxia University, Yinchuan 750021, China. Electronic address: [email protected].
⁹ College of Animal Science and Technology, Ningxia University, Yinchuan 750021, China; Key Laboratory of Ruminant Molecular and Cellular Breeding of Ningxia Hui Autonomous Region, Ningxia University, Yinchuan 750021, China. Electronic address: [email protected].

PMID: 39490705
DOI: 10.1016/j.gene.2024.149044

Abstract

Gene editing technology has become an essential tool for advancing breeding practices, enhancing disease resistance, and boosting productivity in animal husbandry. Despite its potential, the delivery of gene editing reagents into cells faces several challenges, including low targeting efficiency, immunogenicity, and cytotoxicity, which have hindered its wider application in the field. This review discusses the evolution of gene editing technologies and highlights recent advancements in various delivery methods used in animal husbandry. It critically evaluates the strengths and weaknesses of these different delivery approaches while identifying potential directions for future development. The goal is to equip researchers with effective strategies to optimize delivery methods, ultimately facilitating the implementation and progress of gene editing technologies in animal husbandry.

Keywords: Animal husbandry; CRISPR/Cas; Delivery method; Gene editing.

Publication types

Review

MeSH terms

Animal Husbandry* / methods
Animals
CRISPR-Cas Systems*
Gene Editing* / methods
Gene Transfer Techniques*