Whole transcriptome sequencing analysis reveals the effect of circZFYVE9/miR-378a-3p/IMMT axis on mitochondrial function in adipocytes

Int J Biol Macromol. 2024 Nov;281(Pt 2):136916. doi: 10.1016/j.ijbiomac.2024.136916. Epub 2024 Oct 26.

Abstract

Recent research highlights the complex regulation of lipid accumulation and mitochondrial function in adipocytes via non-coding RNAs like microRNAs and circular non-coding RNAs. Circular non-coding RNAs act as endogenous regulators, impacting lipid metabolism and mitochondrial function by interacting with miRNAs. Sequencing white and brown adipose tissues in mice revealed significant variations in 1936 mRNAs, 127 miRNAs, and 171 circRNAs. Analyses showed these RNAs' involvement in vital processes like mitochondrial biogenesis, oxidative phosphorylation, and the citric acid cycle, crucial for lipid metabolism. Focus on top differentially regulated miRNAs led to the construction of a regulatory network involving circRNAs, miRNAs, and mRNAs, illuminating the role of endogenous RNAs in lipid metabolism and mitochondrial function. The circZFYVE9/miR-378a-3p/IMMT axis was identified as influential in adipogenic differentiation of 3T3-L1 preadipocytes by regulating mitochondrial function. This study expands the understanding of non-coding RNAs in adipose tissue, particularly their connection to mitochondrial function and metabolism.

Keywords: Lipid metabolism; Mitochondrial function; ceRNA network.

MeSH terms

  • 3T3-L1 Cells*
  • Adipocytes* / cytology
  • Adipocytes* / metabolism
  • Adipogenesis / genetics
  • Animals
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Gene Regulatory Networks
  • Lipid Metabolism / genetics
  • Mice
  • MicroRNAs* / genetics
  • MicroRNAs* / metabolism
  • Mitochondria* / genetics
  • Mitochondria* / metabolism
  • RNA, Circular* / genetics
  • RNA, Circular* / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Transcriptome / genetics

Substances

  • MicroRNAs
  • RNA, Circular
  • MIRN378 microRNA, mouse
  • RNA, Messenger