Lasy-Seq: A High-Throughput 3' RNA-Seq Method for Large-Scale Transcriptome Profiling in Rice

Natsumi Mori-Moriyama; Atsushi J Nagano

doi:10.1007/978-1-0716-4204-7_13

Lasy-Seq: A High-Throughput 3' RNA-Seq Method for Large-Scale Transcriptome Profiling in Rice

Methods Mol Biol. 2025:2869:123-134. doi: 10.1007/978-1-0716-4204-7_13.

Authors

Natsumi Mori-Moriyama¹, Atsushi J Nagano^{2

3}

Affiliations

¹ Faculty of Agriculture, Ryukoku University, Shiga, Japan.
² Faculty of Agriculture, Ryukoku University, Shiga, Japan. [email protected].
³ Institute for Advanced Biosciences, Keio University, Yamagata, Japan. [email protected].

PMID: 39499473
DOI: 10.1007/978-1-0716-4204-7_13

Abstract

Lasy-Seq (low-cost and easy RNA-seq) is a high-throughput library preparation method for 3' RNA-seq. Usage of oligo(dT) primers with unique index sequences allows simultaneous reverse transcription (RT) reaction and sample indexing. Therefore, each post-RT step, which was performed on a sample-by-sample basis in a conventional method, can be performed on a large number of samples together in a single tube. With the Lasy-Seq, the sequencing-ready library pool can be prepared from a few to several hundred samples in a few days.

Keywords: 3′ RNA-seq; High-throughput RNA-seq; Large-scale RNA-seq; Scalable RNA-seq; Transcriptome.

MeSH terms

Gene Expression Profiling* / methods
Gene Library*
High-Throughput Nucleotide Sequencing* / methods
Oryza* / genetics
RNA, Plant / genetics
RNA-Seq / methods
Sequence Analysis, RNA / methods
Transcriptome

Substances

RNA, Plant