The MAIIA EPO Purification Gel Kit, 7D3-An Alternative Purification Kit for Screening and Confirmation Procedures

D Schwenke; J Hempel; A M Keiler; S C Voss

doi:10.1002/dta.3821

The MAIIA EPO Purification Gel Kit, 7D3-An Alternative Purification Kit for Screening and Confirmation Procedures

Drug Test Anal. 2024 Nov 7. doi: 10.1002/dta.3821. Online ahead of print.

Authors

D Schwenke¹, J Hempel¹, A M Keiler^{1

2}, S C Voss¹

Affiliations

¹ IDAS Dresden, Kreischa, Germany.
² Environmental Monitoring and Endocrinology, Faculty of Biology, Technische Universität Dresden, Dresden, Germany.

PMID: 39511720
DOI: 10.1002/dta.3821

Abstract

In this study, the performance of the new MAIIA EPO purification kit 7D3 was evaluated for human urine samples to confirm compliance with the WADA criteria of the WADA technical document 2024 (TD2024EPO). The kit was validated for selectivity, reliability at minimum required performance levels (MRPLs), limit of detection (LOD), carryover and recovery. A total of 81 urine samples were tested for selectivity, 29 samples for reliability at MRPL, LOD and 16 samples for recovery. The kit fulfils the criteria and the requirements of the upcoming WADA TD2024EPO and presents a valid solution for the purification of ERAs from human urine for doping analyses.

Keywords: EPO; MAIIA; doping; immunopurification.

Grants and funding

Federal Ministry of the Interior, Building and Community (Berlin, Germany)