Molecular and Serological Detection of Mycobacterium tuberculosis in Dairy Cattle From Sylhet, Bangladesh: Implications for Zoonotic Transmission

Md Mukter Hossain; Md Masudur Rahman; Md Mahfujur Rahman; Hemayet Hossain; Ruhena Begum; Md Shahidur Rahman Chowdhury; Md Rafiqul Islam; Md Bashir Uddin

doi:10.1155/2024/3653654

Molecular and Serological Detection of Mycobacterium tuberculosis in Dairy Cattle From Sylhet, Bangladesh: Implications for Zoonotic Transmission

Vet Med Int. 2024 Oct 30:2024:3653654. doi: 10.1155/2024/3653654. eCollection 2024.

Authors

Md Mukter Hossain¹, Md Masudur Rahman², Md Mahfujur Rahman¹, Hemayet Hossain³, Ruhena Begum¹, Md Shahidur Rahman Chowdhury¹, Md Rafiqul Islam¹, Md Bashir Uddin¹

Affiliations

¹ Department of Medicine, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
² Department of Pathology, Faculty of Veterinary, Animal and Biomedical Sciences, Sylhet Agricultural University, Sylhet 3100, Bangladesh.
³ Department of Anatomy and Histology, Sylhet Agricultural University, Sylhet 3100, Bangladesh.

Abstract

This study aimed to determine the seroprevalence and molecular detection of Mycobacterium tuberculosis (M. tuberculosis) in dairy cattle using ELISA and PCR techniques. A total of 500 samples (250 blood and 250 milk) were collected from various farms in Sylhet, Bangladesh. The seroprevalence was found to be 5.6% in blood samples, with PCR confirming 1.60% and 2.80% positivity in blood and milk samples, respectively. These findings highlight the zoonotic potential and public health significance of M. tuberculosis in cattle, suggesting a need for integrated One Health surveillance.

Keywords: Mycobacterium tuberculosis; PCR; cattle; serum ELISA; zoonosis.