Origin of ribonucleotide recognition motifs through ligand mimicry at early earth

RNA Biol. 2024 Jan;21(1):107-121. doi: 10.1080/15476286.2024.2423149. Epub 2024 Nov 11.

Abstract

In an RNA world, the emergence of template-specific self-replication and catalysis necessitated the presence of motifs facilitating reliable recognition between RNA molecules. What did these motifs entail, and how did they evolve into the proteinaceous RNA recognition entities observed today? Direct observation of these primordial entities is hindered by rapid degradation over geological time scales. To overcome this challenge, researchers employ diverse approaches, including scrutiny of conserved sequences and structural motifs across extant organisms and employing directed evolution experiments to generate RNA molecules with specific catalytic abilities. In this review, we delve into the theme of ribonucleotide recognition across key periods of early Earth's evolution. We explore scenarios of RNA interacting with small molecules and examine hypotheses regarding the role of minerals and metal ions in enabling structured ribonucleotide recognition and catalysis. Additionally, we highlight instances of RNA-protein mimicry in interactions with other RNA molecules. We propose a hypothesis where RNA initially recognizes small molecules and metal ions/minerals, with subsequent mimicry by proteins leading to the emergence of proteinaceous RNA binding domains.

Keywords: RNA-world; RRM; Ribonucleotide recognition; aptamers; riboswitch.

Publication types

  • Review

MeSH terms

  • Earth, Planet
  • Evolution, Molecular
  • Ligands
  • Metals / chemistry
  • Metals / metabolism
  • Molecular Mimicry
  • Nucleic Acid Conformation
  • Nucleotide Motifs
  • RNA* / chemistry
  • RNA* / metabolism
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism
  • Ribonucleotides / chemistry
  • Ribonucleotides / metabolism

Substances

  • RNA
  • Ligands
  • Ribonucleotides
  • RNA-Binding Proteins
  • Metals

Grants and funding

The author(s) reported there is no funding associated with the work featured in this article.