Fe3O4 nanoparticles containing gambogic acid inhibit metastasis in colorectal cancer via the RORB/EMILIN1 axis

Cell Adh Migr. 2024 Dec;18(1):38-53. doi: 10.1080/19336918.2024.2427585. Epub 2024 Nov 13.

Abstract

This research aims to study the effect of magnetic nanoparticles of Fe3O4 (MNP Fe3O4) containing gambogic acid (GA-MNP Fe3O4) on colorectal cancer (CRC). MNP Fe3O4 enhanced the antitumor effect of GA by inhibiting the malignant behavior of CRC cells. RORB was a target of GA, and GA activated RORB expression to inhibit metastasis of CRC. Knockdown of RORB impaired the effect of GA-MNP Fe3O4 on CRC metastasis. EMILIN1 was a target of RORB, and RORB promoted transcription of EMILIN1. Overexpression of EMILIN1 reversed the effect of knockdown of RORB on GA-MNP Fe3O4 and inhibited metastasis in CRC. These findings revealed that MNP Fe3O4 enhanced the antitumor effect of GA and activated RORB to promote EMILIN1 transcription and inhibit CRC metastasis.

Keywords: Colorectal cancer; RORB; gambogenic acid; liver metastasis; magnetic nanoparticle.

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Cell Proliferation / drug effects
  • Colorectal Neoplasms* / drug therapy
  • Colorectal Neoplasms* / metabolism
  • Colorectal Neoplasms* / pathology
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Membrane Glycoproteins / metabolism
  • Mice
  • Mice, Nude
  • Neoplasm Metastasis
  • Xanthones* / pharmacology

Substances

  • gambogic acid
  • Xanthones
  • Membrane Glycoproteins

Grants and funding

This study was supported by grants from the Nanjing Health Science and Technology Development Key Program [No. ZKX21028], Provincial Natural Science Foundation of Jiangsu [No. BK20211007], Jiangsu Scientific and Technological Development of Traditional Chinese Medicine Key Projects [No. ZD202227].